For the purpose of pre-implantation genetic diagnosis, blastomer biopsy is conducted on embryos on the 3rd day. Embryos in this period are formed of approximately 6-10 cells (blastomer). For the purpose of genetic examination, 1 or 2 embryos are taken. In order to tekae out a blastomer, a hole should be opened on the outer membrame of the embryo (hatching). For this purpose mechanical, laser or acid tyroid methods are being used.

After this, with biopsy entering from this hole, usually 1 blastomer is taken out without damaging the other blastomers. The blastomer is transferred to the genetic laboratory. FISH or PCR methods are used according to the type of the genetic examination that will be conducted. In couples who will undergo sex determination due to aneuploidy screening, translocation carrying or single gene disease FISH method is used and PCR method is used for ıther gene diseases.

Recently FISH method is being used in our centre. With FISH method, the results are obtained on the same day and normal embryos are transferred on the next day (4th day).

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