During infertility research, one of the most important examinations is spermiogram analysis. “Azoospermia” is a state when no sperm is found during the examinations made before preparation and after washing in spermiogram analysis applied to men a few times on different dates. While sometimes no sperm is found during the examination before washing in sperm analysis, a few sperms can be found during the examination after washing (cryptozoospermia); in such cases, sperm cells can be frozen and preserved to be used at a later time. This saving operation is called “Sperm Pooling”.

Today, one of the most important issues of infertility treatment is to obtain sperm from azospermic men. If sperm production is considered in testis of men as a result of examinations, surveys and evaluations, it is preferred to obtain spermatozoa with open biopsy or aspiration from epididym or testis. The chance of obtaining spermatozoa during operation varies according to the state of patient and results of examinations. If spermatozoa are found in the tissues obtained with these operations, Micro-Injection (ICSI/IMSI) treatment can be applied. Type and reason of Azoospermi should be determined before IVF treatments.

Only ICSI/IMSI can be applied in azoospermic patients. When ICSI/IMSI is applied, fertilization occurs between 45-75%. This value may be lower than the rates obtained with semen. In pairs with Azoospermia, rate of pregnancy is approximately 30-40% and rate of live birth is 25-30%. Fertilization or conventional IVF implementation cannot be performed with sperms obtained from testis or epididym.

After the operation, some of the sperms can be frozen and preserved to be used in the future with the approval of patient if numerical values are appropriate.
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